
Moton Field was constructed in Tuskegee in 1941 as the site for the "Tuskegee Experiment": the U.S. Army Air Corps program to train African American pilots and crewmen during World War II. Built by the students and faculty of the nearby Tuskegee Institute (now Tuskegee University), which had long emphasized vocational education, the site was named in honor of its deceased former president, Robert Russa Moton. The location was chosen because Tuskegee Institute already had a civilian pilot training program, pledged to invest in the development of an airfield, and had engineering and technical advisors and because the region's temperate climate provided ideal flying conditions year round.

On November 6, 1998, Pres. Bill Clinton signed Public Law 105-355 to establish the Tuskegee Airmen National Historic Site at Moton Field. (The legislation had been sponsored in Congress by then Rep. Bob Riley, who represented Alabama's Third Congressional District in which Tuskegee is located.) The site was concurrently placed on the National Register of Historic Places. The new site was projected to contain a museum, interpretive programs, and a national center based on public-private partnership. At the time of the site's grand opening on October 10, 2008, only Hanger One of the two remaining hangars had been restored; the restoration of Hangar Two would be completed some years later.

Outside, a walking trail with several interpretive signs as well as a scenic overlook take visitors around the historic site. In addition to the hangars, there are six other original structures at Moton Field, including the Skyway (Officer's) Club, a storage shed, a warehouse and vehicle storage building, a bath and locker house, a tea room, and a small lunchroom. Two steel skeletal outlines mark the former sites of the cadet house and the Army supply building. Moton Field Municipal Airport is adjacent to the historic site, providing a 5,000-foot runway that accommodates small aircraft and private jets.
According to the National Park Service, more than 30,000 individuals visited the site in 2014. To support the preservation of the memory of the Tuskegee Airmen at Moton Field, the Friends of the Tuskegee Airmen National Historic Site, Inc. was formed in 2009 and works alongside the National Park Service in raising funds and public awareness, providing community outreach activities, and organizing volunteers for special projects. Every year, the Tuskegee Airmen National Historic Site hosts the Tuskegee Airmen Fly-In, which is usually held in May and includes displays of historic aircraft, military fly-bys, aerobatics, exhibits, vendors, and food.
As of February 2016, the Google Arts & Culture Program, in partnership with the U.S. Department of the Interior, has committed to making thousands of National Park Service artifacts available online, including many from National Parks sites in Alabama, including the Tuskegee National Airmen Historic Site. In January 2021, the U.S. Mint released the Tuskegee Airmen National Historic Site Quarter as the last strike of its "America the Beautiful" Quarters Program.