The Huntsville Museum of Art (HMA), located in downtown Huntsville, Madison County, focuses primarily on art by southern artists and works that relate to the region. It currently owns a permanent collection of more than 2,500 pieces that forms the basis of several yearly exhibitions. Typically, the museum sees an average of 80,000 visitors per year.

The museum's core focus is American art, with its permanent collection emphasizing southern art. Representative pieces include works by Kentucky artist Gaela Erwin and Alabama artist and photographer William Christenberry. Other holdings focus on the region, with several relating to Huntsville's connection to the space program, such as Andy Warhol's Moonwalk. In addition to exhibitions arranged from its collection, HMA supports several traveling exhibitions throughout the year. These exhibitions have ranged from catalogues of a single artist such as Maxfield Parrish: The Master of Make Believe to comprehensive exhibits spanning diverse media such as NASA|ART: 50 Years of Exploration.
In 2006, the museum opened an addition, Plaza in the Park, which provides space for Museum Academy classrooms, dining facilities, and the Children's Community Gallery. A notable recent acquisition is The Sellars Collection: Art by American Women, a collection of more than 400 paintings, drawings, and sculptures. In 2010, the museum completed a $7.5 million, 21,000-square-foot expansion that added six new exhibition spaces, an interactive family gallery, attached museum parking, and special event facilities. The museum is funded primarily by private and corporate donations and grants and is governed by the Huntsville Museum of Art Board of Directors.